resistin mouse enzyme-linked immunosorbent assay (elisa) kits  (Cusabio)

Journal: bioRxiv

Article Title: High-fat diet exacerbates the loss of bone fracture toughness in aging for C57BL/6JN mice

doi: 10.1101/2024.08.13.607633

Figure Lengend Snippet: Levels of inflammatory biomarkers: (a) leptin, (b) resistin, (c) vascular endothelial growth factor (VEGF), (d) C-reactive protein (CRP) (e) adipsin, (f) adiponectin, (g) fibroblast growth factor 21 (FGF21), (h) interleukin 4 (IL-4), (i) IL-10, and (j) IL-6. Boxplots illustrate the interquartile range (box), the minimum and maximum values (whiskers), the median value (bar across the box), the mean value (white square), and individual data points (black circles). Significant p-values from 3-way ANOVA and pairwise post-hoc tests are noted on the plots. For all models, the glucose area under the curve (AUC) was considered as a covariate. If the covariate was not significant, the model was rerun without it.

Article Snippet: Measurements included CTX1 (Mouse Cross Linked C-telopeptide of Type I collagen Mini Samples ELISA kit, MyBioSource, San Diego, CA), P1NP ( Mouse procollagen I N-terminal peptide, P1NP ELISA kit (MyBioSource, San Diego, CA), adiponectin (Quantikine ELISA Mouse Adiponectin/Acrp30 kit, R&D Systems, Minneapolis, MN), resistin (Quantikine ELISA Mouse Resistin kit (R&D Systems, Minneapolis, MN), and interleukins 4, 6, and 10 (IL-4, IL-6, IL-10), fibroblast growth factor 21 (FGF21), leptin, vascular endothelial growth factor (VEGF), C-reactive protein (CRP), and complement factor D/adipsin (CFD/adipsin) (Luminex Discovery Assay Mouse Premixed Multi-Analyte kits, R&D Systems, Minneapolis, MN).

Techniques:

Journal: bioRxiv

Article Title: High cholesterol increases myeloma tumour burden and promotes resistance to bortezomib

doi: 10.1101/2025.01.21.633916

Figure Lengend Snippet: ( A ) CD138+ve plasma cells from GSE47552 dataset were analysed using GEO2R and resistin expression is shown. ( B ) Whole bone marrow from bortezomib-responsive and bortezomib-resistant patients (GSE51940 dataset) was analysed using GEO2R and resistin expression is shown. ( C ) Resistin gene expression in 5TGM1, ST2 and ST2-derived BMAds (Adipo). ( D ) Relative resistin gene expression comparing undifferentiated ST2 (ST2) and ST2-derived BMAds (Adipo). ( E ) Resistin protein concentrations secreted by undifferentiated ST2 and ST2-derived BMAds (Adipo). ( F ) Relative resistin expression in BMAds compared to BMAds treated for 3 days with exogenous LDL. ( G ) Relative resistin expression in media from BMAds when treated with LDL or cocultured with 5TGM1 + LDL compared to BMAds on their own. ( H ) 5TGM1 myeloma cell line was cultured alone or cocultured with undifferentiated ST2 or BMAds and resistin gene expression was quantified. For 2 group comparison, two-tailed Student’s t-test was performed. *p< 0.05, **p < 0.01. For more than 2 groups one-way anova analysis was performed. If not otherwise indicated, *p< 0.05, **p < 0.01 & ***p < 0.001. Results are presented as mean ± SEM.

Article Snippet: Resistin concentrations were quantified in bone marrow and serum using mouse resistin ELISA kit (EMRETN, Invitrogen).

Techniques: Expressing, Derivative Assay, Cell Culture, Comparison, Two Tailed Test

Journal: bioRxiv

Article Title: High cholesterol increases myeloma tumour burden and promotes resistance to bortezomib

doi: 10.1101/2025.01.21.633916

Figure Lengend Snippet: ( A ) LDL/VLDL-C ratio in the bone marrow of C57BL/6 KaLwRij mice after 4 weeks of high cholesterol diet. Adipokine analysis in blood ( B ) and bone marrow plasma ( C ). Heat maps show relative expression of adipokines in a non-tumour mouse on cholesterol diet (CHOL), myeloma-bearing mouse (MM) and myeloma-bearing mouse on cholesterol diet (MM CHOL) compared to non-tumour control. ( D ) Relative resistin expression (expressed as fold-increase compared to control) in bone marrow plasma from non-tumour mice fed with control diet (CTR), cholesterol diet for 4 weeks (CHOL 4w) or cholesterol diet for 8 weeks (CHOL 8w). ( E ) Resistin expression in blood from control or cholesterol fed animals for 8 weeks. ( F ) Relative resistin expression (expressed as fold-increase compared to control) in bone marrow plasma in control non-tumour mice (CTR), myeloma-bearing mice (MM) and myeloma-bearing mice on cholesterol diet (MM CHOL) from inoculation. ( G ) Pearson correlation of IgG2bK paraprotein and resistin levels with 17 pairs of XY coming from control and tumour-bearing mice. For 2 group comparison, two-tailed Student’s t-test was performed. For more than 2 groups one-way anova analysis was performed. If not otherwise indicated, *p< 0.05 and ***p < 0.001 compared to control with no-tumour. Results are presented as mean ± SEM.

Article Snippet: Resistin concentrations were quantified in bone marrow and serum using mouse resistin ELISA kit (EMRETN, Invitrogen).

Techniques: Expressing, Control, Comparison, Two Tailed Test